ANSWERS · 4 OCT 2026

As a food business operator in India, how do I choose an FSSAI-notified or NABL-accredited food testing lab, what parameters (nutrition, pesticide residues, microbiology, contaminants) must be tested under FSSAI rules, and what must a food test report contain?

Short answer: Use a lab on FSSAI's current notified list and check that its NABL (ISO/IEC 17025) accreditation is valid on your test date and covers your product and the tests you need. At least once every six months, test the safety parameters for your product category: chemical contaminants, crop contaminants and natural toxins, PCBs/PAH, and microbiology. Add pesticide and veterinary-drug residues and the nutrients you declare on the label where they apply. The report should identify the sample, give the method, result and legal limit for each test, carry the NABL symbol for accredited tests, and be uploaded to FoSCoS.

Approach: I searched FSSAI's laboratory directory, the Contaminants, Toxins and Residues Regulations, the Food Products Standards (Chapter 2) and the labelling amendments. For the licence conditions, the Laboratory and Sample Analysis Regulations, the test-report orders and the Labelling and Display Regulations 2020, I used fssai.gov.in and indiacode.nic.in search snippets, not the full PDFs.

1. Choosing the lab

Who can test

  • Licence Condition 12 lets you test in your own lab, any NABL-accredited lab, or an FSSAI-notified lab (FSSAI licence conditions and FAQs).
  • Under Section 43(1) of the FSS Act, FSSAI may notify labs accredited by NABL or another accreditation body. Recognition requires ISO/IEC 17025 accreditation for the relevant scope from an ILAC/APLAC signatory (FSS Act; FSSAI recognition notice).

What the FSSAI lists contain (current directory)

  • Primary Food Laboratories, Annexure I: 240 entries. Each shows:
    • an FSSAI registration number (e.g. 11/E/FSSAI/2019)
    • the NABL certificate number
    • whether it went through the joint NABL-FSSAI assessment (Y/N)
    • contact details and the Food Analyst
    • separate validity dates for Chemical, Biological, Radiological and Other testing [1]
  • Primary Food Laboratories, Annexure II: 17 entries. The printed validity dates I read there had already passed (e.g. 12 Apr 2025, 22 Oct 2025, 31 May 2026), which matches FSSAI's practice of listing expired accreditations in a separate annexure [2][3].
  • Referral Food Laboratories: 24 entries, e.g. National Food Laboratory Kolkata and Ghaziabad, and NIPHM Hyderabad for pesticide residues [4][5].
  • National Reference Laboratories: each covers a specific domain, e.g. CSIR-IITR for chemical risk assessment and NIFTEM Kundli for fortified foods. EIA Chennai is the ancillary NRL for food microbiology [6][7][8].

Checks before you send samples

  1. Confirm the lab is currently valid. FSSAI publishes monthly validity orders. The 5 June 2026 order lists labs with valid NABL accreditation and, in Annexure II, labs whose accreditation has expired. A July 2026 version also marks labs suspended by NABL or FSSAI.
  2. Check the validity date for the type of testing you need. Some active entries expire soon; one Annexure I lab shows 23 Oct 2026 [9].
  3. Check the detailed scope on the NABL website. The FSSAI list points to it for each lab's detailed scope [2]. Make sure your product category, analyte and method are covered.
  4. A notified lab makes upload easier. Its report can be pulled into FoSCoS from INFOLNET; a report from any other lab is uploaded manually.

2. What must be tested

How often: at least once in six months, as often as your history and risk assessment require. The halves are April to September and October to March, with at least three months between tests. Relabellers count as manufacturers and carry the same duty (Licence Condition 12, FAQs, FoSCoS order).

Parameter groupMinimum under Condition 12?Where the limits are
Metal contaminantsYesReg. 2.1: 11 metals (Pb, Cd, As, Hg, methylmercury, Sn, Cu, Cr, Ni, Sb, Se) [10]. Example: lead in turmeric 10 ppm [11]
Crop contaminants and natural toxinsYesReg. 2.2.1: aflatoxin B1 and total aflatoxins, aflatoxin M1, ochratoxin A, patulin, deoxynivalenol, hydrocyanic acid, agaric acid, hypericine, saffrole. Example: aflatoxin B1 in spices 15 µg/kg [12]
PCBs and PAHYesReg. 2.2: benzo(a)pyrene and the sum of 6 PCBs [13]
MicrobiologyYesAppendix B of the Food Products Standards and Food Additives Regulations. Product clauses refer to it, e.g. cheese, fruit jelly, soup powders, frozen ready-to-eat vegetables [14][15][16]. The Appendix B tables themselves are not in the texts I could read.
Pesticide residuesNot in the snippet's minimum list; test where your raw materials make it relevantReg. 2.3.1 MRL table: 243 substance entries, e.g. wheat MRLs [17]
Antibiotic and veterinary-drug residues (animal and aquatic foods)Test where relevantReg. 2.3.2: 104 compendium rows plus 49 added by Amendment 16 (in force 1 Apr 2025) [18]
Marine biotoxins, histamine, melamineTest where relevantReg. 2.4: PSP, ASP, DSP, AZP and brevetoxin [19]. Reg. 2.5: histamine in fish with a sampling plan (e.g. n=9, c=2, m=100, M=200 mg/kg) [20]; melamine 1.0 mg/kg in powdered infant formula and 2.5 in other foods [21][22]
Composition and qualityProduct standardChapter 2 limits such as moisture and milk fat for cheese [14]
NutritionSupports your label declarationLabelling and Display Regulations 2020: per 100 g/ml (or per single pack) and per serve, declare energy, protein, carbohydrate, total sugars, added sugars, total fat, saturated fat, trans fat and cholesterol. Energy factors are 4/4/9/2 kcal per g (carbohydrate, protein, fat, fibre); protein = Kjeldahl N × 6.25

Upcoming change: Amendment 17 comes into force on 1 Dec 2026. It adds an oxolinic acid limit of 0.3 for seafood and a saffrole limit of 10 for foods containing mace or nutmeg [23][24]. Your October 2026 to March 2027 test cycle should use these limits.

3. What the test report must contain

Statutory formats

  • Official samples are reported on Form VII A (Report of the Food Analyst) or Form XI (Certificate of Analysis, for appeal samples), under the FSS (First Amendment) Rules 2022.
  • FSSAI's 15 Oct 2024 order makes these formats mandatory for notified labs and requires the NABL symbol under NABL 133 for tests inside the lab's accredited scope.
  • Imported food is reported on Form 2 of the Import Regulations 2017.

Contents, per the Laboratory and Sample Analysis Regulations (Forms A and B)

  • Sample details: identification, description and physical appearance, and whether the sample was fit for analysis.
  • The food category and its regulation number.
  • For each parameter: the test, the method used, the result, and the prescribed standard. The standard is the FSS Food Products regulations, or the label declaration for proprietary food.
  • A conclusion, signed by the Food Analyst or the Director of the referral lab.
  • For official samples, the Food Analyst must send the report within 14 days of receiving the sample.

Your own six-monthly reports: I found no official source that prescribes a separate format for them. The practical benchmark is the statutory content above (method, result against the legal limit for each parameter, NABL symbol for accredited tests). The report is then uploaded to FoSCoS under "Upload Six Monthly Lab Testing Report".

If useful, I can pull the full Regulation 2.1, 2.2.1 and 2.3.1 limits for your specific commodity.

Sources:

SOURCES · 24

  1. [1]in_fssai_food_testing_laboratories_directory__primary_annexure_i__s232 · p.23FSSAI
  2. [2]in_fssai_food_testing_laboratories_directory__primary_annexure_ii__s12 · p.24FSSAI
  3. [3]in_fssai_food_testing_laboratories_directory__primary_annexure_ii__s8 · p.24FSSAI
  4. [4]in_fssai_food_testing_laboratories_directory__referral__s24 · p.2FSSAI
  5. [5]in_fssai_food_testing_laboratories_directory__referral__s20 · p.2FSSAI
  6. [6]in_fssai_food_testing_laboratories_directory__nrl_base__s7 · p.2FSSAI
  7. [7]in_fssai_food_testing_laboratories_directory__nrl_supplement__s12 · p.1FSSAI
  8. [8]in_fssai_food_testing_laboratories_directory__nrl_base__s12 · p.2FSSAI
  9. [9]in_fssai_food_testing_laboratories_directory__primary_annexure_i__s237 · p.23FSSAI
  10. [10]Tin in Turmeric whole and powder · p.11FSSAI
  11. [11]Lead in Turmeric whole and powder · p.6FSSAI
  12. [12]Aflatoxin B1 in Spices/Spice Mix · p.14FSSAI
  13. [13]Polychlorinated biphenyls (Sum of PCB28, PCB52, PCB101, PCB138, PCB153 and PCB180) in Marine Fish, Crustaceans and molluscs · p.16FSSAI
  14. [14]2.1.6 · p.7FSSAI
  15. [15]2.3.32 · p.63FSSAI
  16. [16]2.3.39 · p.65FSSAI
  17. [17]Trifloxystrobin in Wheat · p.38FSSAI
  18. [18]Sulfaquinoxaline in All edible animal tissues except in fish · p.25FSSAI
  19. [19]Paralytic Shellfish Poison (PSP) in Bivalve Molluscs · p.60FSSAI
  20. [20]Raw/Chilled/Frozen Finfish in Species with high amount of free histidine (Listed fish species with potential to cause histamine fish poisoning) · p.64FSSAI
  21. [21]Melamine in Powdered infant formula · p.60FSSAI
  22. [22]Melamine in Other foods · p.60FSSAI
  23. [23]Oxolinic acid in Sea foods including shrimps, prawns or any other variety of fish and fishery products · p.6FSSAI
  24. [24]Saffrole in Beverages and foods containing mace and/or nutmeg kernel as an ingredient · p.5FSSAI
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